Randy, thanks for your extensive reply.
I know your articles, I´ve been collecting them and reading.

And about the following...

> David, it's simple math.  Hemacytometer count is simply the average count
per tiny square x 4 million.  You typically count 80 squares.  So one
single spore found among the 80 squares would give an average count of
50,000 spores per bee.  Thus, you'd be unlikely to find even a single spore
if infection level were only 10,000 spores.

Thanks!  Obviously!!...I´m sorry, I´m setting up the lab just now and
I´m still a bit "clumsy" ! If one nosema spore is observed in the
entire central square millimetre grid (25 x 16 = 400), is equal to an
average of 10.000 spores per bee...if level infection´s below 10.000
spores per bee...it´s really difficult find a spore!!


Thanks again

P. David Quesada
Honey Bee Pathology Lab
Extremadura, Spain
http://aym.juntaex.es/organizacion/centros/provincial/zafra/patologiaapicola.htm

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