http://www.tandfonline.com/doi/full/10.1080/00218839.2015.1106777?scroll=top&needAccess=true#.WYpmIIif3zY.mailto
Please check this article in the top. When I mentioned 2 applications, 7 days a part. It is a late fall treatment when brood production is seizing. If the question is how many applications at what intervals does it take to kill mites using OA during brooding period, this is then an entirely different question. I did not do an experiment to answer this question. I expect more than one application is required to cover the period of capped brood till all bees emerge. Generally in our research when we evaluate a product efficacy, we found at least we have to cover 2 periods of capped brood. That is about 32 days to cover capped brood period of drones as well as workers.
We also always use after the treatment using the experimental product such as oxalic acid a finishing treatment. We use a product such as Apivar for 42 -56 days to get the rest of the mites in those previously treated hives then we calculate the difference between mites collected when OA applied And mites collected when Apivar applied. For sure all mites are collected on sticky boards that are replaced every 5 days through the whole period of OA and Apivar application. These mites are counted to provide the numbers to do the math. It is a tremendous work by all means to get the proper answer.
The shared paper mentioned few modes of action on how OA kills mites.
Regards
Medhat
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