Work done at Thessaloniki, Greece, some years ago found that in warmer  
climes 2 x 25g  was sufficient for colonies.
However, it isn't  just the vapor that is important, but also the  
mites coming in contact with the preparation as the bees remove the  
material from the trays and carry it away from the hive.
Those beekeepers who have used Apiguard for some time will notice that  
colonies vary in how much is cleared from the foil trays, which  
suggests that Apiguard is more effective if the bees are good house  
cleaners.
Any material left in the trays and that looks dry can be stirred up  
for further control.

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