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Subject:
From:
Renaud Lavend'Homme <[log in to unmask]>
Reply To:
Informed Discussion of Beekeeping Issues and Bee Biology <[log in to unmask]>
Date:
Mon, 7 May 2018 06:27:19 +0000
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Dear Jon,

> Can someone suggest a minimum RPM for homogenization of germplasm?  
> Looking to order a proper centrifuge.
> look this article :

https://academic.oup.com/jee/article/107/1/47/824301

wegner et al. have tested different speed.(1000, 1300, 1600 g versus 0  
g as a control)

membrane integrity and mobility decrease as speed increases.

I already tested 1300g 12min in BSS diluant  (recipe in the article)

regards

Renaud Lavend'Homme
Belgium

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