>Negative-sense RNA-specific PCR indicated that VDV-1 replicates in the
Varroa synganglion and all other tissues tested (Campbell).

Pete, if you're gonna quote from papers, may I suggest that you include the
entire quotation?  Campbell's full sentence was:
 "Negative-sense RNA-specific PCR indicated that VDV-1 replicates in the
Varroa synganglion and all other tissues tested, but we could not detect
DWV replicating in any Varroa tissue."

So he's saying that DWV-A *was not* replicating in varroa.  Surprisingly,
he's saying that he found evidence that VDV-1 (aka DWV-B) *was* replicating
in the mite.  This despite the findings by Ongus (who first identified
VDV-1) that the only part of the mite in which VDV-1 was found was in the
gastric caecae.

Clearly, due to very conflicting findings, the question does not yet appear
to be answered.  I feel that it's important to know, since DWV-B appears to
have rapidly spread across the U.S. in recent years.  If one form is
evolving to reproduce in two different hosts, then the hypothesis that
hybridization of the two virus strains within the bee, as suggested by
some, could be something that we need to better understand.

-- 
Randy Oliver
Grass Valley, CA
www.ScientificBeekeeping.com

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