From Honey Bee Pests, Predators and Diseases 3rd edition(1997)
edited by Roger A. Morse and Kim Flottum,  Root Publishing
Chapter 4 Protoza Ingemar Fries 
p 72 "To reduce the need of changing combs for control of nosema disease transmission, acetic acid fummigation has been used to kill spores of N.apis on wax combs (Bailey 1957a, Jordan 1957). The acid may be diluted to 60 %, and approximately 2 ml solution/litre volume to be treated used. For treatment, solution is placed on a plate in an empty super on the top bars above a stack of supers. To increase the evapouration area, a crumbled paper or ball of cotton is placed in the acid. The stack of supers is sealed and left undisturbed for at least a week and may be reused after being sufficiently aired."

Chapter 22 Shimanuki and Knox Summary of Control Methods  control of nosema disease P 506
"Acetic acid fumigation
Like heat treatment, acetic acid fumigation renders spore of Nosema apis nonviable. Acetic acid is not approved for this use in the United States. All bees must be removed from the equipment to be treated. The hive bodies with the frames and combs they contain are placed on a floorboard outdoors or in an open shed. A pad of cotton or other absorbent material soaked in 118 ml (1/4 pint) of 80 % acetic acid is placed on the top bars of the frames in each hive. The entrance is blocked and the stack is covered. Cracks in or between the hive bodies should be sealed with tape. The stack should be left undisturbed for one week. After fumigation, the equipment should be aired for about 48 hours before use."

See also Honey Bee Pathology 2nd ed. by Bailey and Ball Acedemic Press 1991

-- Visit www.honeybeeworld.com/bee-l for rules, FAQ and  other info ---