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From:
Georges Prigent <[log in to unmask]>
Reply To:
Informed Discussion of Beekeeping Issues and Bee Biology <[log in to unmask]>
Date:
Thu, 28 Feb 2019 09:14:11 -0500
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<if you can find a single study, other than that by Campbell 2016, that has clearly identified DWV reproduction in any *mite body tissue>


Hi Randy, Pete,
Unless I misunderstand this publication
What about :
S. Gisder, P. Aumeier, E. Genersch Deformed wing virus: replication and viral load in mites (Varroa destructor)
J. Gen. Virol., 90 (Pt 2) (2009), pp. 463-467

Quantification of viral genome equivalents in mites that were collected from symptomatic bees and, therefore, contained replicating virus, had a viral load between 26x10 ^10 and 16x10 ^12 genome equivalents. In contrast, mites from infested pupae that developed into asymptomatic bees contained between 56x10^4 and 16x10^8 DWV genome equivalents….
…Fisher’s least significant difference multiple comparison test (degrees of freedom = 67, MSE=1.793118x10^22, critical value = 1.9960) also showed that a virus titre in mites of 10^10 to 10^12 strongly correlated with viral replication, suggesting that such a high virus titre can only be achieved through replication in mites.
We now provide correlative evidence that this replication produces a high DWV titre in these mites (1010–1012 genome equivalents per mite), which is at least two orders of magnitude higher than the viral titre in mites collected from asymptomatic bees. These results suggested that it is necessary for the DWV titre in the infecting mites to reach a certain threshold level (achieved through replication in these mites) for infested, and hence infected, bees to emerge with crippled wings.
Georges, France

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