Suggestions to thread of where to put apistan
 
Two deeps: I would put one strip per frame of bees as the label reads.
Most of the year this coincides with where the brood is and there are more
mites (80%) in the brood than on adults. As the new adults (bees) emerge
the pregnant female mites are exposed to Apistan, hopefully before they
can scurry into another cell that is ready to be capped.
 
If there is no brood then all the mites are on the adults and strips
should be placed among the bees, whether they are up or down.
 
One deep: Put one strip per five frames where the bees are.  If the bees
are spread evenly throughout all frames then spacing between frame 3
and 4, and between frames 7 and 8 is fine, but if all the bees and brood
are clustered to one side then strips should be evenly spaced in the
cluster.
 
Live long and pollinate, John
 
John A. Skinner           218 Ellington Hall
Extension Apiculturist    University of Tennessee
[log in to unmask]          Knoxville, TN  37901   (423)974-7138