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Wed, 20 Jul 2022 07:48:56 -0700 |
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> The problem with "settling" is that one ends up with honey at the bottom of the tank that is full of particles. What does one do with that? Throw it away? This is why straining is far superior, as even if one uses a settling tank, one still needs to filter the honey from the bottom of that tank..
Here is an image of a clump of material found after me diluting a 10g sample with 100ml of distilled water and then centrifuged at 2500rpm then the supernatant is removed and another 15ml water added and spun again. That was my initial procedure before i started adding 30ml of 99% alcohol and 70ml are warm water to my 1st step to help dissolve the background matrix.
The point is pollen is not equally dispersed and is clumped together with misc debris in a sticky matrix. Heating and pressure filtering would technically be the only way to remove these. Any heating over 50-60C will likely burst the plasma out of the pollen grains.
I use the settling method and keep the last 20 pollen-debris filled jar myself or those who buy it for “allergy” reasons. The top 1” has the most pollen and good stuff. My single strainer removes most of the bee parts. For hobbyists who use buckets with honey valves, the valve is never flush with the bottom of the bucket. The heavies get captured here. This goes into my last jar using a spatula. Nothing goes to waste.
Funny thing is in my 50+ Honey sample observed, only the big chain store brand had bee parts and it had likely been heat filtered as it had next to no pollen. The bee part size tells me it was likely due to post filtering contamination.
Slides 20-24 show post processing honey images. With the exception of the big store brand, the other 3 commercial brands had a healthy number of pollen.
https://drive.google.com/file/d/1Sc9qTC0EJHtNwVb-5GpV5evow-xmay34/view?usp=drivesdk
I used a Omax brand bio microscope outfitted with a digital camera to take my images. The camera is calibrated at each magnifications.
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