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Date: | Mon, 5 Jul 2021 22:26:08 -0400 |
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I haven't posted in a while. Bees are doing good despite the heat wave that made all the way North of 60. Luckily we started the season with lots of rain some the flowering plants have been able to make it so far.
I have also continued building my pollen micrograph reference library now in the 80 species range. I have what I need to start analyzing the samples of honey I have collected for phase 1 (16 Yukon, 3 QC, 2 BC, 1 AK, 2 Commercial brands). I recently received the results from the NMR lab, I just finished compiling the results on a spreadsheet. Over the next month I will conduct my pollen analysis and merge the data.
Some observations from building my pollen ref library and a quick glance at the NMR results:
-Several plant species such as chives and some of the asters popular with other native pollinators shows a wide mix of various pollens, some wind distributed (spruce/pine) but also transferred by those other pollinators. For example the chive flower I collected had about 10 pollen types from completely different flowers (e.g. lowbush cranberry/lingonberry, Jacob's Ladder), some of which were 50 meters from possible sources. One example is lowbush cranberry and Jacob's Ladder which are typically foraged in morning and early afternoon (cooler temps). Chives (growing in our irrigated garden) are visited more often during the warm parts of the day by many solitary bees and bumblebees. I am guessing they are doing this transfer as they mix. I wonder if honey bee foragers also change their target species based on the time of day.
-From my honey pollen analysis trials in developing my preparation procedure, willow seems to be very common in my honeys
-Cheap fine filtered box store pasteurized honeys have very little in them (very boring to look at)
-Labelled honeys (i.e. Blueberry) has very little blueberry pollen in the samples and a lot of everything else
-NMR results, monofloral (labelled fireweed and canola) and the cheap filtered brand have very low Proline numbers
-I will develop an "acid" index to as various types show different types of organic acids. Honeydews have the highest numbers. (possible medicinal values (ref 1)
-I am getting 5 sample re-modelled under the honeydew honey model as the numbers indicate more honeydew than blossom sourced.
- One samples looks like it had traces of sugar syrup ( I did a preliminary pollen analysis on it, very low pollen count overall)
-One of the commercial brands failed on high HMF however very lightly over the allowable limit.
-I attached some of my favourite looking pollen grains
-I developed a pollen counting sheets attached example with made up numbers based on a few references. I added a section so I could use some of the Over/Under represented pollen number factors available in a few studies.
As usual, here is the raw NMR data, I will add the pollen analysis results as I collect it. If you find something interesting, please share with the group. I received public funding so I believe it should be fully open source
https://drive.google.com/file/d/1JW7J4AfvtXA-NglRFv0DOxcdbmw-N2wz/view?usp=sharing
If anyone has interesting study references on honey properties vs origin please send me a note.
Ref 1 - https://www.researchgate.net/publication/265417435_Lactic_acid_bacterial_symbionts_in_honeybees_-_an_unknown_key_to_honey%27s_antimicrobial_and_therapeutic_activities_Lactic_acid_bacteria_a_key_in_honey_production
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