2. Materials and methods
Three batches of each of the following pathogen/parasite solutions were
prepared for the
following treatments: 1) untreated stock solution, not transported nor
irradiated but stored at 4°C until use (stock); 2) stock solution
transported to the irradiation facility without being irradiated 6
(transported) ; and 3) stock solution transported to the irradiation
facility for irradiation (irradiated). The transportation and process of
irradiationwere completed over 5 days in Spring 2016 and over 3 days in
Summer 2017
.
The irradiation treatment was conducted at an USDA/APHIS certified
phytosanitary treatment facility (Gateway America, Gulfport, MS). Stock
solutions were contained in 1.5mL microcentrifuge tubes in cardboard boxes
(transported) or in ziploc bags stapled to the inside wall of a commercial,
wooden Langstroth hive box (irradiated)
.
The transported stock solutions remained in the same warehouse where the
irradiated solutions
were kept and treated that was maintained at 28°C. The reafter, the
transported solutions were brought to the laboratory at the same time as
the irradiated group.
The irradiated stock solutions were exposed to 25kGy of gamma irradiation
delivered over 9 hours 45 minutes, which is a standard dose used for
sanitization purposes (ISO#13409, 2002)
.

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