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Informed Discussion of Beekeeping Issues and Bee Biology <[log in to unmask]>
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Wed, 8 Aug 2018 07:21:41 -0400
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> Ok- can you offer what kind of DNA analysis would confirm and that a colony is Africanized if, for some reason, one needed to know? 

Hi Bill
I know you weren't asking me, but this is something I know a little about. The definitive work is here:

> Current methods of identifying Africanized individuals, such as morphometric analysis and mitochondrial haplotyping, are arguably ineffective and potentially unreliable

> there is no reliable low-cost genetic test for detecting Africanized honeybees available ... it should be possible to develop a low-cost SNP test suitable for use by industry and biosecurity officials. Such tests have been successfully developed to differentiate breeds of swine ...

> This study shows that a panel of 95 single nucleotide polymorphisms, chosen to differentiate between the African, Eastern European and Western European lineages, can detect Africanized honeybees with a high degree of confidence via ancestry assignment. 

Chapman, Nadine C., et al. "A SNP test to identify Africanized honeybees via proportion of ‘African’ ancestry." Molecular ecology resources 15.6 (2015): 1346-1355.

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