Dear Jon,
> Can someone suggest a minimum RPM for homogenization of germplasm?
> Looking to order a proper centrifuge.
> look this article :
https://academic.oup.com/jee/article/107/1/47/824301
wegner et al. have tested different speed.(1000, 1300, 1600 g versus 0
g as a control)
membrane integrity and mobility decrease as speed increases.
I already tested 1300g 12min in BSS diluant (recipe in the article)
regards
Renaud Lavend'Homme
Belgium
http://aristabeeresearch.org
Foundation for breeding varroa resistant honey bees
There can be only one!
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buckfast bee addict -oOOo~(_)~oOOo-
http://perso.fundp.ac.be/~jvandyck/homage/elver/pedgr/ped_RL_2017.html
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