>
> >Do you have more recent information showing that there is both knowledge
> of and ability to choose point of insertion?
>
Christina, I make every effort to post only from a position of factual
knowledge. Many of the fears and questions posed by others in this
discussion could be answered with a minimal internet search. For example,
a few seconds of searching came up with a list of papers such as
Precise gene deletion and replacement using the CRISPR/Cas9 system in human
cells
<http://www.biotechniques.com/BiotechniquesJournal/2014/September/Precise-gene-deletion-and-replacement-using-the-CRISPRCas9-system-in-human-cells/biotechniques-353874.html>
Precise insertion and guided editing of higher plant genomes using Cpf1
CRISPR nucleases <http://www.biorxiv.org/content/early/2017/02/20/109983>
The answer to your specific question is yes--the breeder can choose exactly
where on the DNA strand to insert the gene, thus allowing him/her to
determine when and if it will be "activated."
--
Randy Oliver
Grass Valley, CA
www.ScientificBeekeeping.com
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