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Regarding the infliction of pain, this seems to be a good response:
> The experimental biologist would be advised to follow, whenever feasible, [the] recommendation that insects have their nervous systems inactivated prior to traumatizing manipulation. This procedure not only facilitates handling, but also guards against the remaining possibility of pain infliction and, equally important, helps to preserve in the experimenter an appropriately respectful attitude towards living organisms whose physiology, though different, and perhaps simpler than our own, is as yet far from completely understood. -- Eisemann, C. H., et al. "Do insects feel pain?—A biological view." Experientia 40.2 (1984): 164-167.
Peter L Borst
Ithaca, NY. USA
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