"The close similarity of the results of the CO2 and N2 treatments indicates that the
effects are produced by the same mechanism in both cases. The factor common to
both treatments is that they deprive the bee of oxygen; the effects may therefore be
ascribed to oxygen lack. They are not produced by CHCI3, which acts upon the
nervous system. Dr Wigglesworth, in conversation, has suggested the possibility
that oxygen lack prevents the oxidation of acid metabolites produced by the activity
of the bee, and that temporary high accumulation of these metabolites gives rise in
some way to permanent effects."
My experience with N2 is different. I've used it in the introduction of virgins several times. In those cases I saw bees bringing in pollen the same afternoon that they were smoked with N2. Can't speak to CO@ because I've never used it.
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