>
> > To be clear, under option 1, if you establish four colony
> grades (1, 2, 3, 5) based on metrics such as frames covered by bees,
> frames of
> brood, possibly weight of colony or frames of honey, etc.), and if you
> have
> controls and 4 treatment levels, you'd end up with each control set and
> each treatment composed of a 1, 2, 3, and a 4 grade colony). All of the
> 1s
> would be randomly assigned to the control and to each of four treatments,
> all
> of the 2s would be randomly assigned, etc. There would be 4 control
> colonies, and 4 colonies in each treatment.
Thanks Jerry, totally in agreement, and does not introduce the stress of
homogenization. Homogenization may be useful well in advance of a trial,
though, if one wants to ensure that all colonies contain a background level
of some inoculum, such as nosema. But that could also be done by the
application of a homogenate of ground-up bees taken from all colonies.
Randy Oliver
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