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Subject:
Re: DWV in B Bees & H Bees
From:
Peter L Borst <[log in to unmask]>
Reply To:
Informed Discussion of Beekeeping Issues and Bee Biology <[log in to unmask]>
Date:
Thu, 18 Feb 2010 22:02:32 -0500
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> Has there been any DNA analysis to determine if the honey bee DWV and the bumble bee DWV are the same organism?   Or are they two separate organisms that exhibit the same effect?

It is most definitely the same virus (however, a virus is not an organism). 

> Following sensitive and specific RT-PCR protocols for the detection of DWV in honey bees (Genersch, 2005; Yue and Genersch, 2005), total RNA extracted separately from head, thorax, and abdomen of crippled and asymptomatic bumble bees (RNeasy, Qiagen) was analyzed for the presence of DWV sequences. RNA extracted in the same way from crippled and phenotypically normal honey bees served as controls.  

> All bumble bees (B. terrestris and B. pascuorum) exhibiting deformed wings tested positive for DWV-RNA with all primer pairs in the thorax and abdomen but not in the head. The sequences from all these sources were identical to each other and 99.5% identical to the Italian DWV sequence. These results convincingly demonstrate that the bumble bees analyzed were infected by DWV. DWV infection in bumble bees correlated with wing deformities suggesting that DWV causes crippled wings not only in honey bees but also in bumble bees. 

> DWV is not the only virus described from honey bees that has a broader host range. For example, Kashmir bee virus (KBV) has also been detected in the German wasp (Vespa germanica) (Anderson, 1991), acute bee paralysis virus (ABPV) is also present as inapparent infections in a range of Bombus spp., (Bailey and Gibbs, 1964), and many Apis mellifera viruses and other pathogens–parasites can also infect the other Apis species.

Detection of Deformed wing virus, a honey bee viral pathogen, in bumble bees (Bombus terrestris and Bombus pascuorum) with wing deformities 
Elke Genersch , et al Journal of Invertebrate Pathology 91 (2006) 61–63

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