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Date: | Mon, 2 Aug 2004 17:29:51 +0200 |
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The two main references I use for pollen
identification:
* Methods of melissopalynilogy de
Louveaux (IBRA), look for it on IBRA, you will find
the exact year, page, and so on.
As mentionned by Mr. Huang, you need a centrifugation
after dilution of the honey sample in de-I water. Then
you color the pollen with diluted fushin. Observation
under microscope. I use only the x10x100 combination.
Need also some small basic lab equipment and.... your
own pollen references in gelatine (I mean a local
pollen "hard" data base for comparison).
* Visual identification guide, 1 picture of plant's
pollen under microspcope for each plant:
Sampling and identifying allergenic pollens and molds
by Grant Smith (Blewstone press, San Antonio, Texas)
Local university lab, palynology department, may help
you but their expertise is usually much more in
fossil/sediment samples and they use pollen acetolyse,
a much more complexe procedure that may generate some
pollen distorsion.
Enjoy this pleasant activity !
Hervé
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