Mark,  George was right, of course, in his description of how to diagnose a queenless problem.  Not commented on so far is the effect of elapsed time.  Every day bees are dying as a result of each completing her normal life span.  So as you go through the steps of diagnosis, the population is dwindling.  This is why I am quick to buy a new mated queen instead of waiting to see what happens.  This has happened to me many times and more often than not my new store-bought queen gets killed!  I still do it, though, to make the best use of the population which remains.  Queen breeders must love guys like me!   Dan


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