While in the NICU yesterday, some of the nurses were asking me about the rationale for using expressed breast milk within 24 hours of thawing.  Has this been studied?  Does the milk change in some way or increase in bacterial count after 24 hours?  The nurses wanted to be able to keep the milk in the refrigerator after thawing for 48 hours and I wasn't able to give them a clear reason not to do this, except that the protocols I looked at said 24.  Does anyone know the rationale?

Many thanks,
Anne Nans, RN, IBCLC
Sackets Harbor, NY

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