Content-Transfer-Encoding: |
7bit |
Sender: |
|
Subject: |
|
From: |
|
Date: |
Mon, 2 Apr 2001 06:54:59 EDT |
Content-Type: |
text/plain; charset="US-ASCII" |
MIME-Version: |
1.0 |
Reply-To: |
|
Parts/Attachments: |
|
|
Genetically engineered human lactoferrin is considered by some scientists to
be a broad spectrum antibiotic. Could be worth alot of money.
Valerie W. McClain, IBCLC
1: Biotechnology (N Y) 1995 May;13(5):498-503 <A HREF="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Link&db=PubMed&dbFrom=PubMed&from_uid=9634791">Related Articles, </A><A HREF="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=9634791&dopt=Books">Books, </A><A HREF="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=9634791&dopt=ExternalLink">
LinkOut</A>
A system for production of commercial quantities of human lactoferrin: a
broad spectrum natural antibiotic.
Ward PP, Piddington CS, Cunningham GA, Zhou X, Wyatt RD, Conneely OM
Department of Cell Biology, Baylor College of Medicine, Houston, TX 77030,
USA.
[Record supplied by publisher]
We previously reported the production of limited quantities of biologically
active recombinant human lactoferrin in the filamentous fungus Aspergillus
oryzae. In the present study, we report a modification of this production
system combined with a classical strain improvement program that has enabled
production of levels of recombinant human lactoferrin in excess of 2 g/l. The
protein was expressed in Aspergillus awamori as a glucoamylase fusion
polypeptide which was secreted into the growth medium and processed to mature
human lactoferrin by an endogenous KEX-2 peptidase. The recombinant protein
retains full biological activity in terms of its ability to bind iron and
human enterocyte receptors. Furthermore, the recombinant protein functions as
a potent broad spectrum antimicrobial protein.
PMID: 9634791
***********************************************
The LACTNET mailing list is powered by L-Soft's renowned
LISTSERV(R) list management software together with L-Soft's LSMTP(TM)
mailer for lightning fast mail delivery. For more information, go to:
http://www.lsoft.com/LISTSERV-powered.html
|
|
|