Dear Bee-L people, I have read in an old book the use of chloroform in anaethesising bees, for example when a very vicious but strong colony of bees needs requeening. I have in the past during the course of my undergraduate degree anaethesised Drosophila melanogaster with ether, and I have read in Ashburners book of Drosophila laboratory methods that this can lead to irreversible behavioural changes. These behavioural changes (if they occur) are likely to be more serious in bees than Drosophila living in a milk bottle. I was wondering whether anyone has any experience of any of these methods, and particular in relation to behavioural changes many thanks Steve Pearce Kilspindie Scotland