Subject: "What's he doing NOW?" => Nassanof pheromones lures. (Thanks a lot, Gordon, for your English lesson :-) Hello All ! I prepared my annual provision of tubes containing a Nassanof like compound. It's important to prepare this just at the moment you'll need it because it's volatile and instable. I used a part of the recipe that John B. Free described in his book Social Bee Pheromones, Chapman and Hall, eds, London More details about the discoveries in 2 articles from Boch R and Shearer DA both in Nature(London) : Identification of Geraniol as the active component in Nassanof Pheromone of the Honey Bee Nature 194 (1962) p704-706 Identification of Nerolic and Geranic Acid in the Nassanof Pheromone of the Honey Bee Nature 202 (1964) p320-321 The recipe for 10 tubes: * 1 ml n-hexane * 100 ug (= about 0.1 ml) Citral, mixed isomers (E + Z) * 100 ug (= about 0.1 ml) Geraniol * 100 ug mixed isomers (E+Z) Geranic acid and Nerolic acid As I don't find the last 2 acids, I'm obliged to work without them. Free said it works but not so well that its full formula. Put 0.1 ml of the mixture in a small plastic tube (polyethylene or polypropylene) and close it. I'm using Eppendorf 1.5 ml tubes. A slight lemon like smell! Put the unused tubes in a 'small', 'well closed', 'glas' vessel and put it in the refrigerator or freezer to wait for their use time. Each closed tube is a Nassanof pheromone lure. Geraniol and Citral can be supplied both at the "Sigma Chemical Co" St Louis or at the "Aldrich Co" or at the "ICN" Costa Mesa CA. BTW Does anyone know where it's possible to buy the 2 acids I don't find in my catalogs ? It's important because seems this compounds really increase the efficience of the mixture. TIA Soon ... Jean-Marie <[log in to unmask]>