This is a follow up on a thread I started about a month ago regarding operating a two queen colony. As a quick recap: I had a shallow super brood chamber with a laying queen, over which there were three supers containing Ross Round Section Comb Honey frames. I attempted to sandwich between the second and third honey supers another shallow super brood chamber with another laying queen. Both shallow super brood chambers had queen excluders to keep the queens separated. I was trying to establish a two queen colony to maximize bee production and hence to maximize the round section comb production. Successful section comb production requires a very populous hive to crowd the bees into the section equipment, which the bees work reluctantly if there are more open, less confined spaces available to them. At last report I had not examined the hive to see how blissful was the forced marriage between the two queens, and I have not reported since, as I've been on vacation. Upon my next examination I was unable to find the queen in the sandwiched super, although there were plenty of larvae and sealed brood, which I took as a good sign. It was clear however that when I examined again, the queen in the sandwiched super was no longer present. Fortunately, the queen in the original brood chamber was alive and laying. Long live the queen! Although this was the end of the experiment, it was not the end of the experience. I left the second shallow brood chamber between the second and third comb honey supers to let the brood hatch, hoping to augment the colony's population. Problems arose as the new bees emerged. Since there was no queen laying in the brood chamber, the vacated cells were more to the bees liking for honey production than were the round section supers. By the time most of the bees had hatched out, honey production had moved out of the section equipment and almost exclusively into the now queenless brood super. To deal with this I removed the top section comb super (it had never even been occupied, so it went back to the storage shed). I removed the queenless brood super which was about a third to a half full of honey in only a week!. I shook the bees off each frame onto the ground in front of the hive and gave the queenless, beeless super to another colony to finish filling. It's now almost two weeks since the last manipulations and the colony has fully occupied both of the remaining section comb supers and in fact, if the flow keeps up as it has been going I may need to put the third section comb super back on the hive this weekend. Had it not been for the honey produced in the vacated brood super I thing the bees would already have been well into all three section comb supers. While I was doing all this experimenting, I ordered and read Killion's "Honey in the Comb". It's a great reference for section comb honey production and next year I'll try doing it by the book. As far as a two queen colony goes, it didn't work for me. I may experiment with the idea again some time in the future, but the experiment is over for this year. I hope this has been interesting to the readers of this forum. Aaron Morris - "I think, therefore I bee!"