This is taken from the following site http://www.oda.state.ok.us/main/srvs/agform/checkmite.pdf "Do not treat more than twice a year for varroa mites nor more than four times a year for the small hive beetle. For Varroa Treatment - To control varroa mite, remove honey supers before application of CheckMite+ Strips and do not replace until 14 days after the strips are removed. Use one strip for each five combs of bees in each brood chamber (Langstroth deep frames or equivalent in other sizes). Hang the strips in separate spaces between the combs as near the center of the bee/brood cluster as possible. If two deep brood chambers are used for the brood nest, hang the CheckMite+ Strips in both the top and bottom brood chambers. Treat all infested colonies within the yard. The treatment is most effective when brood rearing is lowest. Effective control may be achieved by treating hives in the spring before the first honey flow and in the fall after the last honey flow. For maximum efficacy leave the strips in the hive for at least 42 days (six weeks). Do not leave the strips in hive for more than 45 days. Do not treat more than twice a year for varroa mites. Honey supers may be replaced 14 days after the strips are removed. For Small Hive Beetle Treatment: To control the small hive beetle, remove honey supers before the application of CheckMite+ Strips and do not replace until 14 days after the strips are removed. Prepare a piece of corrugated cardboard approximately 4x4 inches by removing one side. Remove one CheckMite+ Strip. Cut strip in half crossways and staple the two pieces to the corrugated side of the cardboard. Tape over the smooth side of the cardboard (the side opposite the strips) with duct tape, shipping tape or similar tape to prevent the bees from chewing and removing the cardboard. Or use one sided plastic corrugated sheets. Place cardboard as near the center of the bottom board as possible with the strips down. Make sure the bottom board is clean and the strips lay flat on the bottom board. For maximum efficacy leave the strips in the hive for at least 42 days (six weeks). Do not leave the strips in hive for more than 45 days. Do not treat more than four times per year for the small hive beetle. Honey supers may be replaced 14 days after the strips are removed."