Hi All Just noticed the posts about the CO2 thing. With our bees I have noticed they are far more sensitive to breath at night. The bees become aggressive and run all over the hive with even one small breath in the direction of the hive entrance. A puff of some in the same direction, and then a breath of entance and there is no reaction. My theory - smoke has active particles that bind with receptors in the bees antenae etc temporarily inactivating them. CO2 has little effect except at night when probably other chemicals in our breath make the bees respond. Probably fatty acids or so on (like the ones responbisble for body odour) that will react with the bee receptors that are able to sense such chemicals. Just my theory - the thing to test this would be to make a glass bottle and blow breath through water at the bees. The CO2 could be removed with lime and if the bees still react it is another element of breath. Keep well Garth --- Garth Cambray Kamdini Apiaries 15 Park Road Apis melifera capensis Grahamstown 800mm annual precipitation 6139 Eastern Cape South Africa Phone 27-0461-311663 3rd year Biochemistry/Microbiology Rhodes University Disclaimer: The views and opinions expressed in this post in no way reflect those of Rhodes University.