The information that I give to mothers is 72 hours in the refrigerator. However, our NICU only keeps fresh milk for 48 hours. I don't know what they base this decision on and I guess I will have to ask them at the next breastfeeding resource team meeting. I hadn't given it any thought really. Thank you for jogging my burned out memory. Darlene Breed RN, BSN, IBCLC Coordinator, Milk Bank/Breastfeeding Center Memorial Hospital Worcester, MA