> I cannot answer your question without knowing what kind of behaviour > you are interested in. Some years ago I did some preliminary studies > on using carbon dioxide to knock out bees after a training sequence, > there was no problem with their recovery and rather mixed results as > I recall in the effectiveness of the anesthesia. I've been wondering about the effects of CO2 on bees since I received some package bees from New Zealand that had been packed with bags of dry ice at various places around the pallet. They looked very dopey when they arrived, but were clustered properly. After we installed them, we had some unusual effects, with bees and queens swarming out of brood chambers and combining in huge swarms. This only happened with the portion that we hived within 24 hours of arriving on the plane. The ones we held two days, seemed pretty normal and stayed put. The packages we received from Austrailia exhibited no such behavior, but they had been trucked and stored for several days after coming off the plane. I have wondered if it had anything to do with the CO2, and if it might, if the effects wear off after a day or so. Regards Allen W. Allen Dick, Beekeeper VE6CFK RR#1, Swalwell, Alberta Canada T0M 1Y0 Internet:[log in to unmask] & [log in to unmask] Honey. Bees, & Art <http://www.internode.net/~allend/>