Dear Jon, > Can someone suggest a minimum RPM for homogenization of germplasm? > Looking to order a proper centrifuge. > look this article : https://academic.oup.com/jee/article/107/1/47/824301 wegner et al. have tested different speed.(1000, 1300, 1600 g versus 0 g as a control) membrane integrity and mobility decrease as speed increases. I already tested 1300g 12min in BSS diluant (recipe in the article) regards Renaud Lavend'Homme Belgium http://aristabeeresearch.org Foundation for breeding varroa resistant honey bees There can be only one! \\|// ( o o ) buckfast bee addict -oOOo~(_)~oOOo- http://perso.fundp.ac.be/~jvandyck/homage/elver/pedgr/ped_RL_2017.html /) (\ >80))} {((O8< /) \) (/ >80))} \) *********************************************** The BEE-L mailing list is powered by L-Soft's renowned LISTSERV(R) list management software. For more information, go to: http://www.lsoft.com/LISTSERV-powered.html