> Can someone give the volume of bees necessary to get 300 bees for an alcohol
> wash?
1/2 to 2/3 cup.
> Also, how much alcohol is needed.
1/2 to 2/3 cup.  You can also use windshield washing fluid.
>  Can the alcohol be filtered and reused?
Yes, but it will get progressively dirtier.
>  Is there an optimal
> location in the hive from which to sample the bees?
Bees from a brood frame.  Obviously, avoid using the queen!

Aaron Morris - I think, therefore I bee!

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