>The freezer was airtight?

Pretty close.

>Closing off the air intakes will cause the eggs not to hatch. My guess is
the same would happen with queen cells and especially problematic with
queens ( lack of oxygen)  which hatch and are confined to cell protectors
but I could be wrong.

I've never had a problem.  The more airtight of my freezer/incubators keeps
10 cages of 30 bees healthy, even if I only open every other day.  My guess
it that there is plenty of oxygen either in the incubator for a relatively
small biomass of insects which are not trying to generate heat.

Randy Oliver

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