>The freezer was airtight? Pretty close. >Closing off the air intakes will cause the eggs not to hatch. My guess is the same would happen with queen cells and especially problematic with queens ( lack of oxygen) which hatch and are confined to cell protectors but I could be wrong. I've never had a problem. The more airtight of my freezer/incubators keeps 10 cages of 30 bees healthy, even if I only open every other day. My guess it that there is plenty of oxygen either in the incubator for a relatively small biomass of insects which are not trying to generate heat. Randy Oliver *********************************************** The BEE-L mailing list is powered by L-Soft's renowned LISTSERV(R) list management software. For more information, go to: http://www.lsoft.com/LISTSERV-powered.html Access BEE-L directly at: http://community.lsoft.com/scripts/wa-LSOFTDONATIONS.exe?A0=BEE-L