>Yes, we all use the same methodology. I am not so sure. You told me long ago that you multiply by 50.000 and I said we multiply by 10.000. I haven`t been able to read both Caldwell and Fries papers, but after the seminar by Gabriel Sarlo from Argentina, I understood that if the sample is not homogenous in the Neubauer chamber you count only 5 Thomas reticules and multiply by 50.000, but if the sample is homogeneous you count all of them and multiply by 10.000. I am waiting for the papers to confirm the above. Any one have them?. Particularly Fries Thesis. Cantwell, G.E. (1970). Standard methods for counting nosema spores. American Bee journal, 110:220-223. Fries, I and Ekborn, G. (1984). Nosema apis, sampling techniques and honey yields. Journal of Apicultural Research. 23:102-105 Fries, I.(1988a). Contribution to the study of Nosema disease (Nosema apis Z.) in honey bee (Apis mellífera L.) Swedish University of Agricultural Sciences, Uppsala, Sweden. PhD Thesis. Fries, I.(1988b). Infectivity and multiplication of Nosema apis Z. in the ventriculus of the honey bee (Apis mellífera L.). Apidologie, 19:319-328. -- Juanse Barros J. APIZUR S.A. Carrera 695 Gorbea - CHILE +56-45-271693 08-3613310 http://apiaraucania.blogspot.com/ [log in to unmask] *********************************************** The BEE-L mailing list is powered by L-Soft's renowned LISTSERV(R) list management software. For more information, go to: http://www.lsoft.com/LISTSERV-powered.html Access BEE-L directly at: http://community.lsoft.com/scripts/wa-LSOFTDONATIONS.exe?A0=BEE-L