I have a copy of the photo and the test line was absolutely clear, the control line was a nice strong blue. I have done the EFB test once only, and the result was very faint but was there. I have never had any problem with the AFB test, but I was told by Inspectors in Northern Ireland that they had had false negatives. Ruary -----Original Message----- Gavin said There was absolutely no hint of a 'test' line after the three minutes suggested, a neither was there any 30 mins later. Peter Edwards said Our SBI had similar problems with the AFB kit when conducting a training session for beekeepers. *********************************************** The BEE-L mailing list is powered by L-Soft's renowned LISTSERV(R) list management software. For more information, go to: http://www.lsoft.com/LISTSERV-powered.html