I suppose a small section of queen excluder over the inner hole and a deep with queenless eggs/brood on top of the inner cover could do the trick for a vertical hive?  There would be an exchange of queen scent but it would be much reduced in the top deep.

Waldemar
Long Island, NY

>>Assuming that you do NOT put in any solid division when raising brood to the top, I would expect some pheromone still does get thru - due to interchange of nurse bees between the main queenright nest and the separated brood due to some bees travelling down the hive for cleansing flights for example - and also the pheromone coating on the brood frmames would only 'wear out' over time, not be suddenly expunged.

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