Medhat Nasr said: > High levels of coumaphos (Average= 45.53 ppm, n= 8) were found > in wax samples from colonies where failing queens were found > and subleathal toxicity to bees were observed. Wax samples (n=8) > collected from good active colonies had an average of 23.66 ppm > coumaphos. I saw your abstract, and had a few questions about it. Can you confirm that all the colonies from which the samples were taken were treated strictly per instructions? I ask because any talk of unusual levels of contamination are often met with the claim that such levels must have been the result of "misuse" or "unapproved formulations". (Great marketing strategy, eh? Blame your customers.) > Coumaphos was found in 83% of honey samples with a range >3-8 ppm. When you say "honey samples", do you mean jars of final product, or specific samples of honey taken from brood comb areas? In 2000, the US EPA set a tolerance for coumaphos in honey at 0.1 ppm (one tenth part per million, aka "100 parts per billion"). http://www.honey.com/pressrm/research/coumaphos.html If the "samples" were harvested honey, does this mean that over half of coumaphos users can expect to harvest honey that is from 30 to 80 times the US "legal limit"? (Say it ain't so, please!) Even if the "samples" were taken from the brood area, what are the odds that the same few drops of honey could be moved to the supers, harvested, and end up being "sampled" during a honey test? (OK, it is a bit of a long shot, but who wants to bet their entire crop on it?) The US EPA tolerance for coumaphos in wax is a whopping 100 ppm. (See the same link, above) a) If 45 ppm in wax is associated with queen failures b) and 23 ppm does not seem to have overt short-term effects One starts plotting curves in one's head of how long before foundation made from recycled wax becomes universally toxic to bees. I don't use coumaphos, but from your numbers, I must conclude that I may soon be unknowingly exposing my bees to coumaphos simply by buying foundation. One hopes that the makers of foundation will "wake up" to this problem, and start testing wax before they use it to make foundation, but this will eliminate the "trade your wax for foundation" game, as smaller lots of wax are not "worth" the expense of such testing. jim :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: -- Visit www.honeybeeworld.com/BEE-L for rules, FAQ and other info --- ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::