Hi Allen and Everyone, My exposure to the sugar shake test were limited to the procedures proscribed by it's authors. My own experience with using powdered sugar involved treating whole hives of bees and watching the mite fall on a mite trap. Some sampling was done until I was confident of the effects and observations of individual bees were made. I not sure what the effects of rolling bees around in a jar of sugar for 15 minutes would be. My own observations indicate that extrapolating infestation rates from one small sample, regardless of the technique used to liberate the mite from the bee, is not very accurate especially at moderate to low levels of infestation. That's why I hit the whole hive with powdered sugar. It's much more accurate. Give it a test and let us know what happens. Best Wishes Dennis