United States Patent 5,571,896
Conneely , et al. November 5, 1996
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Production of recombinant human lactoferrin
Abstract
The present invention provides novel plasmids, transfected eucaryotic cells
and methods of producing these plasmids and transfected eucaryotic cells. The
novel plasmid contains the cDNA for human lactoferrin protein. Methods for
the production of human lactoferrin protein in A. Oryzae are also provided.
Thus, the present invention provides an efficient and economical means for
the production of recombinant human lactoferrin protein.
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Inventors: Conneely; Orla M. (Houston, TX); Headon; Denis R. (Galway, IE);
O'Malley; Bert W. (Houston, TX); May; Gregory S. (Houston, TX)
Assignee: Baylor College of Medicine (Houston, TX)
Appl. No.: 250308
Filed: May 27, 1994
U.S. Class: 530/400; 530/395; 530/412; 435/69.1; 435/69.7; 435/259.11;
435/254.2; 435/254.21; 435/254.23; 435/254.3; 435/320.1; 536/23.4; 536/23.5;
536/24.1; 935/10; 935/27; 935/28; 935/37; 935/48; 935/68; 935/69
Intern'l Class: C07K 014/79; C12N 015/12; C12N 015/62; C12N 015/80
Field of Search: 536/23.5,23.4,23.74
435/254,254.11,320.1,69.1,254.21,254.23,254.3,254.22,254.2,69.7
530/394,395,350
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References Cited [Referenced By]
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U.S. Patent Documents
4652639 Mar., 1987 Stabinsky 435/91.
4740461 Apr., 1988 Kaufman 435/69.
5155037 Oct., 1992 Summers 435/240.
5304633 Apr., 1994 Tomita et al. 530/326.
Foreign Patent Documents
PCT/US87/00119 Jan., 1987 WO.
PCT/DK88/00245 Sep., 1988 WO.
WO91/13982 Sep., 1991 WO 530/400.
Other References
Epstein, J. B., et al., Reviews of Infectious Diseases, vol. 6, No. 1, "Oral
Candidiasis: Pathogenesis and Host Defense", pp. 96-106. 1984.
Soukka, T., et al., FEMS Microbiology Letters, vol. 90, "Fungicidal effect of
human lactoferrin against Candida albicans", pp. 223-228 (1992).
M. Metz-Boutigue et al. "Human lactotransferrin: amino sequence and
structural comparisons with other transferrins" Eur. J. Bioch., 145:659-676
(1984).
B. Anderson et al.; "Structure of Human Lactoferrin: Crystallographic
Structure Analysis and Refinement at 1-8 A Resolution" J. Mol. Biol.
209:711-734 (1989).
K. Stowell et al.; "Expression of cloned human lactoferrin in baby-hamster
kidney cells" Biochem. J., 276:349-355 (1991).
P. Mead et al.; "cDNA and protein sequence of bovine lactoferrin" Nucleic
Acids Research, 18:7167, No. 18 (1990).
M. Rey et al.; "Complete nucleotide sequence of human mammary gland
lactoferrin", 18:5288, No. 17 (1990).
M. Powell et al.; "Nucleotide sequence of human lactoferrin cDNA" Nucleic
Acids Research, 18:4013, No. 13 (1990).
B. Pentecost et al.; "Lactotransferrin Is the Major Estrogen Inducible
Protein of Mouse Uterine Secretions" J. Biological Chem., 262:10134-10139,
No. 21 (1987).
T. Rado et al.; "Isolation of Lactoferrin cDNA From a Human Myeloid Library
and Expressions of mRNA During Normal and Leukemic Myelopoiesis" Blood,
70:989-993, No. 4 (1987).
A. Pierce et al.; "Molecular cloning and sequence analysis of bovine
lactotransferrin" Eur. J. Biochem., 196:177-184 (1991).
Valenti et al. 1986, FEMS Microbiology Letters 33:271-275.
Promego, 1988/89, Price List, 2800 S. Fish Hatchery Road, Madison, WI.
Huge-Jensen et al. 1989. Lipids, 24(9): 781-785.
Siner et al. 1989. Gene, 79:107-117.
von Heyne, G. 1984, J. Mol. Biol. 173:243-251.
Primary Examiner: Wax; Robert A.
Assistant Examiner: Moore; William W.
Attorney, Agent or Firm: Halluin; Albert P. Pennie & Edmonds
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Goverment Interests
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This invention was made with government support under Grant No. HD 27965
awarded by the National Institute of Health. The government has certain
rights in the invention.
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Parent Case Text
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This is a continuation, of application Ser. No. 07/873,304, filed Apr. 24,
1992, now abandoned.
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