Bob, thanks for the response on the quantity of liquid nitrogen per 
frame and the precautionary tips.  After sending my message, I found 
Dick and Jane instructions on the web for "Testing Honey Bee Colonies 
for Hygienic Behavior" using liquid 
nitrogen. 
http://www.extension.org/pages/Testing_Honey_Bee_Colonies_for_Hygienic_Behavior

I have a healthy respect for toxic and/or hazardous chemical, as I'm 
a graduate entomologist with a minor in chemistry, a certified pest 
control operator and developed the US Army hazardous waste storage 
and disposal program.  While on active duty, I evaluated hazardous 
waste and pest management programs at many Army installations.

I have a line of survivor bees which were a cut-out from a tree in 
2000 and they have never been treated any chemical.  Except for an 
unusual wet or dry Spring and early Summer, each colony will produce 
an average of 150 pounds of honey.  The bees are gentle requiring 
little smoke to work them.  Of the 10 colonies, I very seldom lose a 
colony or have to requeen them.  In the past two years, one colony 
became queenless, perhaps a virgin queen failed to return from a 
mating flight, and one colony chimneyed up through the two honey 
super to the top, freezing to death head-first in cells with frames 
of honey on both sides.  At the present time, I have 10 colonies of 
this line of bees, each with about 100 pounds of honey 
stores.  Hopefully, they will all make it through the Winter and Spring.

I want to test this line of bees to determine how hygienic they are; 
as, they continue to survive varroa and other pests and diseases 
without any treatments.

Jim Young
McAlester, OK

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