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Subject:	Re: PCR Primers for Africanized bees?
From:	Peter Loring Borst <[log in to unmask]>
Reply To:	Informed Discussion of Beekeeping Issues and Bee Biology <[log in to unmask]>
Date:	Thu, 10 Nov 2016 19:57:52 -0500
Content-Type:	text/plain
Parts/Attachments:	text/plain (14 lines)

A 485-bp section of the cytochrome b gene was amplified using the primers 5 -TAT GTA CTA CCA TGA GGA CAA ATA TC-3  and 5 -ATT ACA CCT CCT AAT TTA TTA GGA AT-3  developed by Crozier et al. (1991).

291- and 194-bp fragments obtained for A. m. mellifera, A. m. ligustica, A. m. syriaca, A. m. cypria, A. m. lamarckii, A. m. caucasia, A. m. carnica, and A. m. iberiensis. 485-bp fragment obtained for A. m. iberiensis, A. m. intermissa, and A. m. scutellata.

Mitochondrial DNA characterization of Africanized honey bee (Apis mellifera L.) populations from the USA
Journal of Apicultural Research and Bee World 49(2): 177-185 (2010) © IBRA 2010

PLB

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