You will have to get the queen into the observation hive, then the others will
stay. She is probably in a separate little cage, among the bees. You could fish
out the little cage, then pour or allow the bulk of the bees to fall into the
observation hive ( an open top is necessary for this) then open the little cage
and allow the queen to walk down among the throng. (Don't let the queen climb up
and fly as you open her cage) Once the queen and most of the  bees are in there,
allow the remaining bees access to the entrance (lay the package near the
entrance) and they will locate the  entrance by scent.
Good luck.