Varroa will die in I believe seven days without a host.

Sounds to me like you have got fluvalinate resistant varroa *if* you treated
with Apistan last spring. If so Apistan is not going to work.

Sounds like your varroa load is over threshold. If so the hive is beyound
saving and wasting money on.

Do a 24 hour varroa mite natural drop and report back for my opinion.

email me direct if you want.

Bob

Ps. reinfestation could be taking place but I suspect fluvalinate resistant
varroa are the problem but you could observe at the entrance for about 15
minutes and see if bees with varroa are landing.
or
Close the entrance and eyther roll a couple hundred of the bees which
congregate at the blocked entrance.

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