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From:
Adony Melathopoulos <[log in to unmask]>
Reply To:
Informed Discussion of Beekeeping Issues and Bee Biology <[log in to unmask]>
Date:
Wed, 26 Nov 2003 10:37:13 -0500
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Just out in Apidologie this month is a paper from the UK regarding the
combination of oxytetracycline treatments and shaking bees onto
foundation for the management of EFB.  The abstract is below.  Below the
UK abstract is a second abstract from 2001 regarding shaking bees onto
foundation without antibiotics for the management of AFB.

ABSTRACT 1
Controlling European foulbrood with the shook swarm method and
oxytetracycline in the UK
Ruth J. Waite, Michael A. Brown, Helen M. Thompson and Medwin H. Bew
National Bee Unit, Central Science Laboratory, York, YO41 1LZ, UK

Abstract
Colonies infected with European foulbrood (EFB) were treated with the
shook swarm method in combination with oxytetracycline (OTC) and
compared with those treated with OTC alone, the usual treatment for EFB
in England and Wales. Success rates and instances of recurrence in the
following season were recorded in the seasons 2000 and 2001
respectively. Both treatments had similar success rates with respect to
elimination of EFB in 2000. Shook swarm plus OTC treatment resulted in a
lower level of EFB recurrence in the 2001 season than OTC treatment
alone. Colonies treated with the shook swarm plus OTC method showed a
recurrence rate of 4.8%, whereas those treated with OTC alone had a
recurrence in 21.1% of cases. The differences were shown to be
significant at the 10% level. These results suggest that the shook swarm
plus OTC method could be a valid method for EFB treatment and control in
the UK.

ABSTRACT 2
Effect of shaking honey bee colonies affected by American foulbrood on
Paenibacillus larvae larvae spore loads

 Journal of Apicultural Research,   August 2001, vol. 40, no. 2,   pp.
65-69(5)

Del Hoyo M.L.; Basualdo M.; Lorenzo A.; Palacio M.A.; Rodriguez E.M.;
Bedascarrasbure E.

Abstract:
Honey bee (Apis mellifera) colonies exhibiting clinical symptoms of
American foulbrood (AFB, causative agent Paenibacillus larvae larvae)
were divided into 2 groups that received different shaking treatments:
(1), shaking adult bees into a new hive; and (2), shaking adult bees in
front of the entrance to a new hive. Honey bee and honey samples were
taken before shaking and 1, 22, 44 and 66 days after shaking.
Microbiological cultures were made from honey and honey bee samples to
determine P. l. larvae development. The average number of P. l. larvae
cfu/g honey before shaking was 89.86 ± 17.93 (mean ± s.e.) and more than
500 cfu/bee for honey bee samples. Honey bee samples had more
colony-forming units before shaking but differences were not
statistically significant after shaking. An important reduction in the
number of colony-forming units in honey bee and honey samples was
detected after shaking by both methods and no significant difference was
detected between them. Honey and honey bee samples were positive for the
presence of P. l. larvae in every sampling but no AFB clinical symptoms
were detected in the honey bee colonies after 5 months. These results
allow us to conclude that both shaking methods reduce spore loads in new
colonies without using chemicals and, using it with other management
procedures would allow development of an integrated AFB control method.

Adony

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