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Subject:
Re: Nursing protects honeybee larvae from secondary metabolites of pollen
From:
randy oliver <[log in to unmask]>
Reply To:
Informed Discussion of Beekeeping Issues and Bee Biology <[log in to unmask]>
Date:
Mon, 11 Jun 2018 07:53:28 -0700
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>
> >I have a class of 40 online Master Beekeepers looking for projects this
> week.


I had a visiting beekeeper from Chile yesterday, so we looked at the guts
of some 5th instar larvae.
Protocol: uncap newly-sealed cells, gently remove the larva with forceps.
Crush the larva and stir the body fluids.
Add cover slip.
Look under a scope at 400x--scanning across for pollen grains.

Some have zero, some (which typically show yellow when crushed) show some
grains.
In some larvae, there are obviously digested pollen grains.
But in no larvae have I yet seen enough grains, compared to the amount in a
nurse bee gut, to provide much protein nutrition.

I'd be interested in confirmation of this from others.


-- 
Randy Oliver
Grass Valley, CA
www.ScientificBeekeeping.com

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