From:
CHANGES IN THE BEHAVIOUR OF HONEY-BEES
FOLLOWING THEIR RECOVERY FROM ANAESTHESIA
BY C. R. RIBBANDS
-- begin quote ---
Another effect of CO 2 upon the honey-bee has already been recorded, by Macken-
sen (1947). He showed that queen honey-bees which have been instrumentally
inseminated commence laying a week or more earlier after CO 2 anaesthesia than they
do after some other anaesthetics, and that uninseminated virgins subjected to
similar treatment are soon caused to lay unfertilized eggs. Mackensen's results are
complementary to those of Janisch (1924). Janisch exposed to CO 2 for short periods
(less than 1 min) the females of three species of grain pests; he concluded that these
insects were 'artificially aged', so that they went through their potential mating
period, and that the treatments thus made them sterile.
--- end quote ---
This is very interesting as I understand that Juanse uses N20 to introduce virgins with
good success.
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