We were given two kits to demonstrate at Gormanstown, we actually read the
instructions which say that a larva suspected of being diseased is to be
removed intact and transferred to the bottle of buffer solution.

(I do not think that scale material or would necessarily work as I think
that the bacteria  is supposed to be in the vegetative state, not
sporulated.)

This is then shaken for 20 seconds and three drops placed in the test.

One line shows the test is running, if there is a second line, no matter how
faint within three minutes the test is positive.

We did the tests on Wednesday and Thursday, the sample of comb was removed
from the hive on the previous Thursday, so as you may imagine any larvae
present were not in good condition.

Both test were positive ( the lines were very faint).

Microscope slides made from the scale in the combs showed the spores.
----- Original Message -----
Subject: Re: [BEE-L] Vita's new field testing kit for AFB


>
> Yes, but this was not a small sample from a hive.  It was dug out of
several
> cells in a heavily
> infected comb - confirmed, although no-one would have needed it, by the
NBU
> laboratory.  The cells had all the classic symptoms - perforated cappings,
> scale etc.

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