Equipment:

Pint jar with ring.
8th inch hardware cloth insert in ring instead of lid.
100 honeybees.
Level tablespoonful of confectionary sugar (powdered sugar).
Shallow container (white Saucer, works well) with water, if desired.

Collect approximately 100 honeybees and place in jar, affix screen
lid.

Place powdered sugar on screen and rub it in, roll jar to coat bees
with powdered sugar, set aside for 5 minutes.

Shake sugar with any dislodged mites onto a white surface for
counting.

Better yet, shake into the shallow container of water. The sugar will
dissolve, and the dislodged mites will be floating on the surface of
the water for ease of counting.

Release bees for return to there home.

This method has proven most effective, is easy to use and does not
kill bees. I never liked the idea of killing bees.

Doc