To add to the previous thoughts, I wonder how to best test whether using N20
is better/worse than other options.

The simplest, I'm thinking would be to make up 10 similar nucs and treat 5 with 
N2O and confine the other 5 for three days before opening them.  Then observe 
them for a month or two.

This would compare practical results of the two mthods and that is what really 
matters. 

A keen observer could go further and count brood, flights, etc., but if the nucs 
work out the same for all intents and purposes, that is enough for most of us.

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