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Subject:
From:
James Fischer <[log in to unmask]>
Reply To:
Informed Discussion of Beekeeping Issues and Bee Biology <[log in to unmask]>
Date:
Tue, 13 Jan 2015 08:29:03 -0500
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Whasamatter?  
Can't tell one plant's pollen from another, even with color photomicrographs
of 250 kinds of pollen and that new microscope Santa brought?

Well, fret no more, give those tired eyes a rest - a little PCR work, a new
sequencer, and some open-source analysis software like Sumatra and
Sumaclust, and viola - "DNA-meta-barcoding" will ID the plant species from
which those pollen grains where forged!

http://www.sciencecodex.com/a_honey_bee_hive_tells_all-148699
http://tinyurl.com/obokk4v

But wait!  There's more!  If you act now, you might even avoid all the mess,
bother, and waiting of PCR, and go straight to "shotgun sequencing"!

http://www.gigasciencejournal.com/content/2/1/4
http://tinyurl.com/kcdkao3

The call it "next-generation sequencing", as opposed to traditional "Sanger
sequencing", but this is clearly a misnomer, as if the gear is available
now, it would be "current-generation".   A better description would be
"parallel sequencing", as one can do 30 or more genes at a time.  But this
is starting to sound a lot like arguments over video-game systems and
computers.  (Funny, my artist friends never bicker over which brand of
paintbrush is superior.)

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