It sure looks like you pulled all the triggers I would have pulled and more. When I have had what I presumed was EFB terra treatments showed a positive response in a few days. I treated three times as per the label. I have not had a single case that reappeared after treatment. I also have only found it in nucs that were under five frames of bees.
You also have done experiments that show it is limited to this years queens. I would lean hard on the queen supplier for information. I also would not buy his queens again for a while.
Dick
" Any discovery made by the human mind can be explained in its essentials to the curious learner." Professor Benjamin Schumacher talking about teaching quantum mechanics to non scientists. "For every complex problem there is a solution which is simple, neat and wrong." H. L. Mencken
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